VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

TRIS-EGG YOLK-GLYCEROL DILUENT/UNIVERSAL DILUENT

Composition

Tris-Egg yolk-Glycerol diluent is called as Universal diluent.

S.No.	Components	Quantity
1	Tris	3.028 gm
2	Citric acid	1.675 gm
3	Fructose	1.250 gm
4	Glycerol	7 ml
5	Penicillin G sodium	1 lac unit
6	Dihydrostreptomycin	100 mg
7	Distilled Water	Make up to 80 ml
8	egg yolk	20 ml

• Inclusion of glycerol at room temperature was found as satisfactory as 5⁰C.
• Tris dilutor allows filling and sealing of poly vinyl straws at room temperature while freezing the semen.

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• Preparation of Tris-egg yolk- glycerol diluent

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