Biochemical Techniques and Instrumentation

2.2. Gel filtration chromatography

Gel filtration chromatography is also known as size exclusion chromatography or permeation chromatography or molecular sieve chromatography. In this technique, the separation of molecules is achieved based on their molecular size. The molecular sieve properties of a variety of porous gel materials are utilized for the separation of molecules. The dextran beads are first porous gel materials introduced for the separation of biological molecules such as polysaccharides, proteins, peptides and glycoproteins.