Biochemical Techniques and Instrumentation

2.2.2.3. Polyacrylamide gels

Polyacrylamide gels are formed by polymerization of acrylamide and methylene bis acrylamide. They are stable in aqueous buffers at pH 1-10. They withstand higher pressures and have lower susceptibility to bacterial contamination.They are supplied as a dry powder and swollen prior to use. They are commercially available as Bio-Pel P gels. The fractionation ranges of polyacrylamide gels are given in Table 2.3.2.3.

Gel type	Fractionation range (Da)
P 2	100 - 1,800
P 4	800 - 4,000
P 6	1,000 – 6,000
P 10	1,500 – 20,000
P 30	2,500 – 40,000
P 60	3,000 – 60,000
P 100	5,000 – 1,00,000
P 200	30,000 – 2,00,000
P 300	60,000 – 4,00,000