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 Course overview |
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 Syllabus |
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Lecture Plan |
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Course overview |
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Syllabus |
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Lecture Plan |
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| Topic 1 |
Learning objectives |
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Definition for Biotechnology |
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Branches of Biotechnology |
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Colour codes for branches of Biotechnology |
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Animal Biotechnology |
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Objectives of Animal Biotechnology |
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Applications of animal biotechnology |
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Transgenic technology |
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Gene knockout technology |
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Molecular genetics |
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Embryo transfer technique |
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In vitro embryo production |
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Modern vaccines |
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Molecular diagnostics |
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Nutritional biotechnology |
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 PODCAST |
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Learning objectives |
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Definition for Biotechnology |
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Branches of Biotechnology |
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Colour codes for branches of Biotechnology |
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Animal Biotechnology |
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Objectives of Animal Biotechnology |
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Applications of animal biotechnology |
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Transgenic technology |
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Gene knockout technology |
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Molecular genetics |
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Embryo transfer technique |
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In vitro embryo production |
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Modern vaccines |
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Molecular diagnostics |
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Nutritional biotechnology |
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PODCAST |
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| Topic 2 |
Learning objectives |
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Prokaryotes |
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Eukaryotes |
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Differences between Prokaryote and Eukaryote |
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General Organization of eukaryotic cells |
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Proteins and Nucleic acids |
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Polysaccharides |
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Lipids |
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Complex macromolecules |
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Lipoproteins |
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Glycolipids |
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PODCAST |
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Learning objectives |
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Prokaryotes |
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Eukaryotes |
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Differences between Prokaryote and Eukaryote |
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General Organization of eukaryotic cells |
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Proteins and Nucleic acids |
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Polysaccharides |
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Lipids |
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Complex macromolecules |
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Lipoproteins |
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Glycolipids |
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PODCAST |
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| Topic 3 |
Learning objectives |
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Composition of Nucleic acid |
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Purines and Pyrimidines |
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Structure of Purines and Pyrimidines |
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Base pairing between nitrogenous bases |
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PODCAST |
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Learning objectives |
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Composition of Nucleic acid |
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Purines and Pyrimidines |
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Structure of Purines and Pyrimidines |
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Base pairing between nitrogenous bases |
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PODCAST |
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| Topic 4 |
Learning objectives |
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DNA structure |
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DNA Replication |
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RNA structure |
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Transcription |
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Transcription in Eukaryotes |
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PODCAST |
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Learning objectives |
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DNA structure |
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DNA Replication |
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RNA structure |
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Transcription |
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Transcription in Eukaryotes |
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PODCAST |
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| Topic 5 |
Learning objectives |
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The genetic code |
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Codon-anti codon interaction |
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Initiator tRNA |
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Mechanism of protein synthesis |
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Initiation |
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Elongation |
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Termination |
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Translation in Eukaryotes |
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Post-translational modifications |
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Wobble hypothesis |
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PODCAST |
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Learning objectives |
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The genetic code |
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Codon-anti codon interaction |
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Initiator tRNA |
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Mechanism of protein synthesis |
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Initiation |
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Elongation |
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Termination |
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Translation in Eukaryotes |
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Post-translational modifications |
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Wobble hypothesis |
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PODCAST |
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| Topic 6 |
Learning objectives |
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Recombinant DNA |
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Recombinant DNA technique |
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Purposes of recombiant DNA technique |
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Restriction Enzymes |
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Uses of restriction enzymes |
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Vector, DNA insert and Host |
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Steps in recombinant DNA technique / gene cloning |
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Applications of recombinant DNA technology |
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PODCAST |
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Learning objectives |
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Recombinant DNA |
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Recombinant DNA technique |
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Purposes of recombiant DNA technique |
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Restriction Enzymes |
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Uses of restriction enzymes |
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Vector, DNA insert and Host |
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Steps in recombinant DNA technique / gene cloning |
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Applications of recombinant DNA technology |
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PODCAST |
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| Topic 7 |
Learning Objectives |
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Cloning vector |
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Features of cloning vectors |
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General steps in cloning |
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Types of cloning vectors |
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Plasmids |
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Phages |
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Cosmids |
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Bacterial artificial chromosome |
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Yeast artificial chromosome (YAC) |
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PODCAST |
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Learning Objectives |
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Cloning vector |
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Features of cloning vectors |
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General steps in cloning |
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Types of cloning vectors |
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Plasmids |
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Phages |
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Cosmids |
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Bacterial artificial chromosome |
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Yeast artificial chromosome (YAC) |
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PODCAST |
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| Topic 8 |
Learning objectives |
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Expression vectors |
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Expression systems |
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Prokaryotic protein expression systems |
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Eukaryotic protein expression systems |
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Expression vectors with strong promoters |
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Inducible expression vectors |
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Expression vectors that produce fusion proteins |
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Uses of recombinant DNA technology in human health |
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Uses of recombinant DNA technology in Animal science |
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Uses of recombinant DNA technology in agriculture |
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PODCAST |
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Learning objectives |
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Expression vectors |
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Expression systems |
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Prokaryotic protein expression systems |
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Eukaryotic protein expression systems |
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Expression vectors with strong promoters |
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Inducible expression vectors |
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Expression vectors that produce fusion proteins |
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Uses of recombinant DNA technology in human health |
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Uses of recombinant DNA technology in Animal science |
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Uses of recombinant DNA technology in agriculture |
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PODCAST |
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| Topic 9 |
Learning objectives |
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Transformation |
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Competent cells |
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Preparation of E.coli competent cells |
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DNA transformation |
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Transfection |
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Methods |
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Stable and transient transfection |
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RNA transfection |
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PODCAST |
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Learning objectives |
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Transformation |
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Competent cells |
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Preparation of E.coli competent cells |
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DNA transformation |
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Transfection |
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Methods |
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Stable and transient transfection |
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RNA transfection |
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PODCAST |
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| Topic 10 |
Learning objectives |
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Polymerase Chain Reaction |
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Components of PCR |
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PCR cycles |
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Types of PCR |
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Applications of PCR |
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PODCAST |
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Learning objectives |
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Polymerase Chain Reaction |
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Components of PCR |
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PCR cycles |
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Types of PCR |
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Applications of PCR |
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PODCAST |
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| Topic 11 |
Learning objectives |
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DNA libraries |
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Genomic library |
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 Steps in Genomic DNA library |
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Applications of Genomic library |
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PODCAST |
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Learning objectives |
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DNA libraries |
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Genomic library |
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Steps in Genomic DNA library |
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Applications of Genomic library |
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PODCAST |
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| Topic 12 |
Learning objectives |
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cDNA library |
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Preparation of cDNA library |
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Preparation of cDNA library |
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Uses of cDNA library |
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PODCAST |
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Learning objectives |
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cDNA library |
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Preparation of cDNA library |
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Preparation of cDNA library |
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Uses of cDNA library |
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PODCAST |
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| Topic 13 |
Learning objectives |
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DNA sequencing |
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Methods of DNA sequencing |
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Maxam-Gilbert Method |
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Sanger's Dideoxy method |
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DNA sequencing |
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Automated sequencing |
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Steps in automated cycle sequencing |
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Separation of molecules |
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Detection on an automated sequencer |
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Assembling of sequenced part of a gene |
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DNA sequencing - Electropherogram |
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RNA sequencing |
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PODCAST |
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Learning objectives |
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DNA sequencing |
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Methods of DNA sequencing |
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Maxam-Gilbert Method |
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Sanger's Dideoxy method |
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DNA sequencing |
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Automated sequencing |
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Steps in automated cycle sequencing |
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Separation of molecules |
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Detection on an automated sequencer |
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Assembling of sequenced part of a gene |
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DNA sequencing - Electropherogram |
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RNA sequencing |
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PODCAST |
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| Topic 14 |
Learning objectives |
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Southern blotting |
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Steps in southern blotting |
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Southern blotting |
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Southern blotting2 |
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Southern blotting3 |
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Applications of Southern blotting |
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PODCAST |
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Learning objectives |
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Southern blotting |
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Steps in southern blotting |
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Southern blotting |
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Southern blotting2 |
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Southern blotting3 |
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Applications of Southern blotting |
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PODCAST |
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| Topic 15 |
Learning objectives |
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Western blotting |
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Steps in Western blotting |
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Western blotting |
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Applications of Western blotting |
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Northern blotting |
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Steps in Northern blotting |
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Northern blotting |
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Applications of Northern blotting |
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Differences between Southern blotting and Northern blotting |
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PODCAST |
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Learning objectives |
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Western blotting |
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Steps in Western blotting |
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Western blotting |
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Applications of Western blotting |
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Northern blotting |
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Steps in Northern blotting |
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Northern blotting |
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Applications of Northern blotting |
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Differences between Southern blotting and Northern blotting |
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PODCAST |
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| Topic 16 |
Learning objectives |
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Probes and its types |
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Probe labeling |
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Different ways of generating Probes |
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Learning objectives |
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Probes and its types |
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Probe labeling |
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Different ways of generating Probes |
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Isotopic and non-isotopic labeling systems |
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Advantages and Disadvantages of hot and cold probes |
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Uses of DNA probes |
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PODCAST |
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| Topic 17 |
Learning objectives |
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DNA finger printing |
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Methods of DNA finger printing |
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RFLP |
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RFLP |
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PCR |
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AFLP |
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AFLP |
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STR |
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PODCAST |
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| Topic 18 |
Learning objectives |
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Embryo Transfer In Cattle |
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Selection of donors |
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Induction of superovulation |
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Superovulation |
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Superovulation2 |
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Schedule for superovulation in cattle |
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Embryo collection |
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Embryo collection |
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Selection of Recipients |
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Transfer of embryos |
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Embryo transfer |
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Cryopreservation of embryos |
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Applications of embryo transfer |
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PODCAST |
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| Topic 19 |
Learning objectives |
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In vitro Fertilisation (IVF) |
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Oocyte recovery |
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In vitro culture and maturation of oocytes |
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Preparation of spermatozoa |
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In vitro fertilization in farm animals |
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In vitro fertilization |
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Sexing of Embryos |
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Methods |
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Micromanipulation |
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Advantages and disadvantages of microinjection |
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Animal cloning |
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Animal cloning |
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PODCAST |
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| Topic 20 |
Learning objectives |
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Transgenic animals |
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Steps in prodction of transgenic animals by DNA micro injection |
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Steps in Transgenic animal production |
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Applications of tansgenic animals |
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Uses of Transgenic animals |
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Biopharming |
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Need for biopharming |
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Criteria to select a plant for biopharm production |
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Benefits of biopharms |
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Risks of biopharms |
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PODCAST |
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| Topic 21 |
Learning objectives |
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Genome mapping |
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Methods of genome mapping |
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Physical mapping |
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Genome sequencing |
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Methods of genome sequencing |
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BAC to BAC sequencing |
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Whole genome shotgun method |
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Whole genome sequencing |
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PODCAST |
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| Topic 22 |
Learning objectives |
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Marker Assisted Selection |
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Molecular markers |
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Techniques used for marker assisted selection |
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Restriction fragment length polymorphism |
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Simple sequence repeats |
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Single nucleotide polymorphism |
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Gene bank |
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Purpose of Gene banking |
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Programmes of gene banking |
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PODCAST |
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| Topic 23 |
Learning objectives |
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Animal nutrition biotechnology |
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DNA technologies in nutritional physiology |
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DNA technologies in rumen biology |
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PODCAST |
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| Topic 24 |
Learning objectives |
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Need for rumen manipulation |
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Steps to optimize rumen digestion |
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organisms |
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Application of recombinant DNA technology to rumen organisms |
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PODCAST |
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| Topic 25 |
Learning objectives |
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Cell culture and cell lines |
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Primary cell culture |
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Cell lines |
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Regularly used cell lines |
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Products obtained from cell lines |
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PODCAST |
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| Topic 26 |
Learning objectives |
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Tumor markers |
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Importance of Tumor markers |
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Useful tumor markers |
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Acute phase proteins (APP) |
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Synthesis and biological functions of APPs |
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Applications of acute phase proteins |
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PODCAST |
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| Topic 27 |
Learning objectives |
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Applications of PCR in disease diagnosis |
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Applications of DNA probe/DNA hybridization in disease diagnosis |
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PODCAST |
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| Topic 28 |
Learning objectives |
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Monoclonal antibodies |
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Steps in production of Monoclonal antibodies |
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Monoclonal antibody production |
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Applications of monoclonal antibodies |
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PODCAST |
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| Topic 29 |
Learning objectives |
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Vaccines |
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Subunit vaccines |
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Creating a subunit vaccine |
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Advantages and disadvantages of subunit vaccine |
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Recombinant DNA vaccine |
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Recombinant Vaccines for Animal Use |
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Recombinant Vectored Vaccines |
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New generation vaccines |
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DNA vaccine |
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PODCAST |
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| Topic 30 |
Learning objectives |
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Fermentation technology |
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Upstream and Down stream processes in fermentation |
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Types of fermenters |
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Fermenters |
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Fermentation process |
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Applications of fermentation technology |
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PODCAST |
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| Topic 31 |
Learning objectives |
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Milk fermentation process |
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Fermented milk products |
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Yogurt |
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Sour cream |
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Kefir |
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Cheese |
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Meat fermentation |
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Fermented sausages |
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Biotechnological processes in leather making |
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PODCAST |
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| Topic 32 |
Learning objectives |
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Ethical issues in Biotechnology |
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Food safety |
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Environmental Considerations |
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Regulations for Animal Biotechnology |
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PODCAST |
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| Topic 33 |
Learning objectives |
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Intellectural properties |
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Categories of intellectual property |
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Industrial property |
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Copyright |
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Patents |
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Conditions of patentability |
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Product patent and Process patent |
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Pantentee and Exclusive rights |
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Trade mark |
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Collective marks |
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Certification marks |
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Trade names |
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Geographical indications |
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World Intellectual Property Organization (WIPO) |
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PODCAST |
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| Topic 34 |
Learning objectives |
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Bioinformatics |
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Components of bioinformatics |
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Importance of computers in bioinformatics |
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Important fields of bioinformatics |
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Structural bioinformatics |
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Drug designing |
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Phylogenetics |
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Computational biology |
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Population genetics |
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Splicing site prediction |
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miRNA prediction |
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RNA structure prediction |
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Gene prediction |
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Transcription factor binding site prediction |
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Genome annotation |
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Ancestry prediction |
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PODCAST |
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| Topic 36 |
Isolation of Lymphocyte |
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Isolation of lymphocytes |
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| Topic 37 |
Chromosome spread preparation and staining |
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Chromosome spread preparation |
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| Topic 38 |
Superovulation |
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Superovulation |
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Superovulation2 |
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| Topic 39 |
Collection and Recovery of Embryos |
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Embryo collection |
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| Topic 40 |
Embryo transfer |
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Embryotransfer |
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| Topic 41 |
Chicken Embryo Fibroblast culture |
Chicken Embryo Fibroblast culture |
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| Topic 42 |
Production of monoclonal antibodies |
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Monoclonal antibody production |
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| Topic 43 |
DNA Sequencing – Sanger's method (Dideoxy method) |
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DNA sequencing - Sanger's method |
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| Topic 44 |
Polymerase chain reaction |
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Amplification of target DNA by PCR |
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| Topic 45 |
Agarose Gel Electrophoresis |
Agarose Gel Electrophoresis |
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Agarose electrophoresis |
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| Topic 46 |
Isolation of Plasmid DNA from E.coli |
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Isolation of Plasmid DNA |
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| Topic 47 |
Isolation of RNA |
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Isolation of Total RNA |
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| Topic 48 |
Restriction Enzyme Analysis |
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Restriction enzyme analysis |
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| Topic 49 |
Cloning paper plasmid |
Cloning Paper Plasmid |
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| Topic 50 |
Detection of Infectious bronchitis virus by RT-PCR |
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| Topic 51 |
Question bank |
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Suggested books |
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Useful links |
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Acknowledgement |
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Participants