Week	Name	Description
	Theory
	Practical
18 February - 24 February	1.1.1. Introduction
	1.1.2. Interaction of EMR with matter
	1.1.3. UV-Vis Spectroscopy
	1.1.4. Basic laws of light absorption
	1.2.1. UV-Vis Spectrophotometer
	1.2.2. Double beam UV-Vis Spectrophotometer
	1.2.3. Light source
	1.2.3.Monochromators
	1.2.5. Sample cell
	1.2.6. Detectors
	Spectrum measurement
	1.2.7. Applications
	Theory and Applications of Spectrophotometry
25 February - 3 March	2.1. Basic principles of chromatography
	2.1.1. Chromatographic separation
	2.1.2. Types of chromatographic system
	2.1.3. Equilibrations
	2.1.4. Applications
	Principles of Chromatography
	2.2. Gel filtration chromatography
	2.2.1.Principle
	2.2.2. Column matrices
	2.2.2.1.Dextran gels
	2.2.2.2. Agarose gels
	2.2.2.3. Polyacrylamide gels
	2.2.2.4. Composite gels
	2.2.3. Chromatography equipment
	2.2.3.1. Columns
	2.2.3.2. Pumps
	2.2.3.3. Fraction collectors
	2.2.3.4. Detectors
	2.2.4. Technique
	Gel filtration chromatography
	2.2.5. Applications
	Gel Filtration Chromatography
	Fractionation Ranges of Popular Agarose-Based Separation Materials
	Fractionation Ranges of Popular Composite Separation Materials
	2.3.1. Ion Exchange Chromatography
	2.3.2. Principle
	2.3.3. Types of Ion exchangers
	2.3.4. Ion exchanger support matrix
	2.3.4.1. Polystyrene
	2.3.4.2. Cellulose
	2.3.4.3. Sepharose and Sephadex
	2.3.4.4. Choice of ion exchanger
	2.3.5. Eluent buffers
	2.3.6. Elution
	2.3.7. Technique
	Ion exchange chromatography
	2.3.8. Applications
	Ion exchange chromatography
	2.4. Affinity chromatography
	2.4.1. Principle
	2.4.2. Affinity chromatography support matrices
	2.4.2.1.Organic supports
	2.4.2.2. Inorganic supports
	2.4.2.3. Synthetic supports
	2.4.2.4. Composite supports
	2.4.3. Types of ligands
	2.4.4. Immobilization methods
	2.4.5. Spacer arms
	2.4.7. Elution techniques
	2.4.8. Applications
	Affinity chromatography
	Ligands table 2.5.3
	2.6. Thin Layer Chromatography
	2.6.1. Principle
	2.6.2. Adsorbents
	2.6.3. Plate preparation
	2.6.4. Technique
	2.6.5. Retention factor
	2.6.6. Quantification
	2.6.7. Preparative TLC
	2.6.8. Two dimensional TLC
	2.6.9. Applications
	Thin Layer Chromatography
	2.6. Gas Chromatography
	2.6.1. Two techniques
	2.6.2. Principle
	2.6.3. Instrumentation
	2.6.3.1. Carrier gas
	2.6.3.2. Injector
	2.6.3.3. Column
	2.6.3.3.1. Packed column
	2.6.3.3.2. Capillary column
	2.6.3.4. Stationery phase
	2.6.3.4.1. Polysiloxanes
	2.6.3.4.2. Polyethylene glycols
	2.6.3.4.3. Cross-linked and bonded phases
	2.6.3.5. Thermostatic oven
	2.6.3.6. Detection
	2.6.3.6.1. Flame Ionization Detector (FID)
	2.6.3.6.2. Electron Capture Detector (ECD)
	2.6.3.6.3. Flame Photometric Detector (FPD)
	2.6.3.6.4. Thermal Conductivity Detector (TCD)
	2.6.3.7. Amplifier and Integrator
	2.6.4. Applications
	Gas Chromatography
	2.7. High Performance Liquid Chromatography
	2.7.1. Principle
	2.7.2 Instrumentation
	2.7.2.1. Mobile phases
	2.7.2.2. Pumping System
	2.7.2.2.1. Types of pumps
	2.7.2.3. Sample Injection System
	2.7.2.4. Column
	HPLC
	2.7.2.4.1. Stationery phase
	2.7.2.4.2. Guard column
	2.7.2.5. Detectors
	2.7.3. Applications
	High Performance Liquid Chromatography
4 March - 10 March	3.1.1. Radioisotopes
	3.1.2. Radioactive decay
	3.1.3. Types of radioisotopes
	3.1.4. Applications
	3.1.5. Radioimmunoassay
	3.1.6. Technique
	3.1.6.1. Separation of bound antigen from free antigen
	3.1. 7. Applications
	Radio Isotopes - Radio Immuno Assay
	3.2.1.Enzyme Linked Immuno Sorbent Assay (ELISA)
	3.2.2. Technique
	3.2.3. Components of ELISA
	3.2.4. Classification of ELISA
	3.2.4.1. Direct ELISA
	Direct antigen capture
	Direct antibody capture
	3.2.4.2. Indirect ELISA
	Indirect antigen ELISA
	Indirect antibody ELISA
	3.2.4.3. Competitive ELISA
	3.2.4.4. Reverse ELISA
	3.2.5. ELISA Plate Reader
	3.2.6. Applications
	Enzyme Linked Immuno Sorbent Assay
11 March - 17 March	4.1.1. Introduction
	4.1.2. Basic Principles of Sedimentation
	4.1.3. Types of centrifuges
	4.1.4. Differential centrifugation
	4.1.5. Density gradient centrifugation
	4.1.6. Rotor categories
	4.1.7. Selection of Centrifuge Tubes
	4.2.1. Ultracentrifugation
	4.2.1.1. Preparative ultracentrifuge
	4.2.1.2. Analytical ultracentrifuge
	4.2.1.2.1. Kinds of experiments
	4.2.3. Uses of ultracentrifuge
	Ultracentrifugation
	Classification of centrifuges based on the speed of rotation
18 March - 24 March	5.1.1. Introduction
	5.1.2. Southern Blotting
	5.1.3. Blotting Techniques
	5.1.4. Applications
	5.2.1.Northern Blotting
	5.2.2. Blotting Technique
	5.2.3. Applications
	5.3.1. Western Blotting
	5.3.2. Blotting Technique
	5.3.2.1. Tissue preparation
	5.3.2.2.Gel electrophoresis
	5.3.2.3. Transfer
	5.3.2.4. Blocking
	5.3.2.5. Detection
	5.3.2.5.1. Methods of Detection
	5.3.4. Applications
	Blotting - Southern, Northern and Western
25 March - 31 March	6.1.1. Introduction
	6.1.2. Technique
	6.1.3. Components of PCR
	6.1.4. PCR reaction
	6.1.5. Steps in PCR
	PCR
	6.1.6. Detection
	6.1.7.Applications
	Polymerase Chain Reaction
	6.2.1. Introduction
	6.2.2. History
	6.2.3.Types of cloning
	6.2.3.1. Molecular cloning
	6.2.3.2. Cellular cloning
	6.2.3.3. Organism cloning
	6.2.3.3.1. Embryo cloning
	6.2.3.3.2. Reproductive cloning
	6.2.3.3.2.1. Roslin technique
	6.2.3.3.2.2. Honolulu technique
	6.2.3.3.2.3. Problems in reproductive cloning
	6.2.3.3.3. Therapeutic cloning
	6.2.3.3.3.1. Hurdles in therapeutic cloning
	6.2.4. Advantages and Disadvantages of Cloning
	DNA Cloning
	Cloning
	6.3.1. Introduction
	6.3.2. Types of cultures
	6.3.3. Steps in Cell Culture
	6.3.3.1. Isolation of cells
	6.3.3.2. Maintenance of cells
	6.3.3.3. Manipulation of cultured cells
	6.3.4. Applications
	6.4.1. Introduction
	6.4.2. Technique
	6.4.3. Applications
	Hybridoma technology
1 April - 7 April	Aim
	Beer’s Law
	Lamberts Law
	Materials
	Procedure
	Observation
8 April - 14 April	Aim
	Analysis of Total Free and Protein Bound Sugars by Spectrophotometer
	Principle
	Materials
	Procedure
	Protein Bound Carbohydrates
	Calculation
15 April - 21 April	Aim
	Analysis of protein by Spectrophotometer (Lowry's Method)
	Principle
	Materials
	Procedure
	Calculation
22 April - 28 April	Aim
	Determination of Histamine by Spectrofluorometric method
	Principle
	Materials
	Procedure
	Calculation
29 April - 5 May	Aim
	Materials
	Procedure
6 May - 12 May	Aim
	DNA Quantification by Spectrophotometer
	Materials and Procedure
	Calculation
13 May - 19 May	RNA Quantification by Spectrophotometric Determination
	Procedure
	Comments
20 May - 26 May	Aim
	Identification of Amino acids in Fish by Paper Chromatography
	Principle
	Materials
	Procedure
	Calculation
27 May - 2 June	Aim
	Determination of fatty acids of fish by gas chromatography
	Materials
	Procedure
	Calculation
3 June - 9 June	Aim
	Procedure
	Gel filtration technique
	Materials
10 June - 16 June	Southern Blotting Technique
	Reagents
	Procedure
	PreHybridization Reaction
	[32P]-Labelling of Probe
	Hybridization
	Stripping Southern Blot on Nitrocellulose
	Procedure
	Reagents
	Southern Blot Protocol
17 June - 23 June	Aim
	Western Blotting Technique
	Reagents
	Procedure
24 June - 30 June	Qualitative and quantitative analysis of DNA by Agarose Electrophoresis
	Agarose electrophoresis - Principle
	Procedure
1 July - 7 July	Aim
	Detection of Salmonella by PCR
	Equipments
	Materials
	Procedure
8 July - 14 July	Aim
	Subcellular fractionation by Centrifugation
	Reagents
	Procedure
15 July - 21 July	Aim
	Detection of antigen by Enzyme-linked immunosorbent assays (ELISAs)
	Materials
	Procedure
	ALP substrate
22 July - 28 July	Aim
	Isolation of Plasmid DNA by centrifugation
	Reagents
	Procedure
	Analysis
29 July - 4 August	Aim
	SDS-PAGE Separation of Proteins
	Principle
	Materials
	Procedure
	Documentation
12 August - 18 August	References